Chemical methods for the simultaneous quantitation of metabolites and proteins from single cells

J Am Chem Soc. 2015 Apr 1;137(12):4066-9. doi: 10.1021/jacs.5b00944. Epub 2015 Mar 19.

Abstract

We describe chemical approaches for integrated metabolic and proteomic assays from single cells. Quantitative assays for intracellular metabolites, including glucose uptake and three other species, are designed as surface-competitive binding assays with fluorescence readouts. This enables integration into a microarray format with functional protein immunoassays, all of which are incorporated into the microchambers of a single-cell barcode chip (SCBC). By using the SCBC, we interrogate the response of human-derived glioblastoma cancer cells to epidermal growth factor receptor inhibition. We report, for the first time, on both the intercellular metabolic heterogeneity as well as the baseline and drug-induced changes in the metabolite-phosphoprotein correlation network.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Cell Line, Tumor
  • Drug Screening Assays, Antitumor / instrumentation*
  • Drug Screening Assays, Antitumor / methods
  • Equipment Design
  • ErbB Receptors / antagonists & inhibitors
  • Erlotinib Hydrochloride / pharmacology
  • Fluorescent Antibody Technique / instrumentation
  • Fluorescent Antibody Technique / methods
  • Glioblastoma / drug therapy
  • Glioblastoma / metabolism
  • Humans
  • Metabolomics / instrumentation*
  • Metabolomics / methods
  • Microarray Analysis / instrumentation*
  • Microarray Analysis / methods
  • Proteomics / instrumentation*
  • Proteomics / methods

Substances

  • Antineoplastic Agents
  • Erlotinib Hydrochloride
  • ErbB Receptors