The effect of mycophenolic acid on epigenetic modifications in lupus CD4+T cells

Clin Immunol. 2015 May;158(1):67-76. doi: 10.1016/j.clim.2015.03.005. Epub 2015 Mar 17.

Abstract

Systemic lupus erythematosus (SLE) is a complex systemic autoimmune disease involving multiple organs and characterized by overproduction of autoantibodies and T and B cell abnormalities. The treatment for SLE has been restricted to immunosuppressants and corticosteroids. Mycophenolate mofetil (MMF), as a relatively new immunosuppressant, is now widely used in the treatment of SLE patients, particularly those with nephritis. However, it is unclear whether mycophenolic acid (MPA) could modulate the reported disorders of epigenetic status in CD4(+)T cells from SLE patients. In this study, we demonstrated that MPA can upregulate the histone H3/H4 global acetylation status by regulating HATs and HDACs in lupus CD4(+)T cells. Furthermore, we found that MPA also affected the histone H4 acetylation and histone H3K4 tri-methylation levels in CD40L promoter region that inhibited the expression of CD40L. These findings indicate the potential epigenetic mechanism of therapeutic effects of MPA in SLE.

Keywords: CD4(+)T cells; CD40L; Epigenetics; Mycophenolic acid; Systemic lupus erythematosus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation / drug effects
  • Adult
  • CD11a Antigen / drug effects
  • CD11a Antigen / metabolism
  • CD27 Ligand / drug effects
  • CD27 Ligand / metabolism
  • CD4-Positive T-Lymphocytes / drug effects*
  • CD4-Positive T-Lymphocytes / immunology
  • CD40 Ligand / drug effects
  • CD40 Ligand / metabolism
  • Case-Control Studies
  • Cells, Cultured
  • DNA Methylation / drug effects*
  • Enzyme Inhibitors / pharmacology*
  • Epigenesis, Genetic / drug effects*
  • Epigenesis, Genetic / immunology
  • Female
  • Histone Deacetylases / drug effects
  • Histone Deacetylases / genetics
  • Histone Deacetylases / metabolism
  • Histones / drug effects*
  • Histones / metabolism
  • Humans
  • Lupus Erythematosus, Systemic / immunology*
  • Methylation / drug effects
  • Middle Aged
  • Mycophenolic Acid / pharmacology*
  • RNA, Messenger / drug effects*
  • RNA, Messenger / metabolism
  • Young Adult

Substances

  • CD11a Antigen
  • CD27 Ligand
  • Enzyme Inhibitors
  • Histones
  • RNA, Messenger
  • CD40 Ligand
  • Histone Deacetylases
  • Mycophenolic Acid