A simple PCR-based fluorometric system for detection of mutant fusion DNAs using a quencher-free fluorescent DNA probe and graphene oxide

Chem Commun (Camb). 2015 Apr 25;51(32):6960-3. doi: 10.1039/c5cc00263j. Epub 2015 Mar 23.

Abstract

We propose a facile fluorometric system for detection of gene mutations using graphene oxide (GO). A fluorescent probe DNA anneals to a specific mutant gene and is degraded by the 5'→ 3' exonuclease activity of Taq polymerase during PCR, and the released fluorophore retains fluorescence after addition of GO without quenching.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Probes / chemistry*
  • Fluorescent Dyes / chemistry*
  • Fluorometry / methods*
  • Fusion Proteins, bcr-abl / genetics
  • Graphite / chemistry*
  • Mutation*
  • Oxides / chemistry*
  • Polymerase Chain Reaction
  • Taq Polymerase / metabolism

Substances

  • DNA Probes
  • Fluorescent Dyes
  • Oxides
  • Graphite
  • Fusion Proteins, bcr-abl
  • Taq Polymerase