The Wnt effector transcription factor 7-like 2 positively regulates oligodendrocyte differentiation in a manner independent of Wnt/β-catenin signaling

J Neurosci. 2015 Mar 25;35(12):5007-22. doi: 10.1523/JNEUROSCI.4787-14.2015.

Abstract

Genetic or pharmacological activation of canonical Wnt/β-catenin signaling inhibits oligodendrocyte differentiation. Transcription factor 7-like 2 (TCF7l2), also known as TCF4, is a Wnt effector induced transiently in the oligodendroglial lineage. A well accepted dogma is that TCF7l2 inhibits oligodendrocyte differentiation through activation of Wnt/β-catenin signaling. We report that TCF7l2 is upregulated transiently in postmitotic, newly differentiated oligodendrocytes. Using in vivo gene conditional ablation, we found surprisingly that TCF7l2 positively regulates neonatal and postnatal mouse oligodendrocyte differentiation during developmental myelination and remyelination in a manner independent of the Wnt/β-catenin signaling pathway. We also reveal a novel role of TCF7l2 in repressing a bone morphogenetic protein signaling pathway that is known to inhibit oligodendrocyte differentiation. Thus, our study provides novel data justifying therapeutic attempts to enhance, rather than inhibit, TCF7l2 signaling to overcome arrested oligodendroglial differentiation in multiple sclerosis and other demyelinating diseases.

Keywords: BMP signaling; TCF7l2(TCF4); canonical Wnt/beta-catenin signaling; myelination; oligodendrocyte differentiation; remyelination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Morphogenetic Proteins / physiology
  • Cell Differentiation / genetics
  • Cell Differentiation / physiology*
  • Gene Expression Regulation, Developmental / genetics
  • Gene Expression Regulation, Developmental / physiology*
  • Gene Knockdown Techniques
  • Mice
  • Mice, Transgenic
  • Myelin Sheath / genetics
  • Myelin Sheath / physiology
  • Oligodendroglia / cytology*
  • Oligodendroglia / physiology*
  • Signal Transduction / genetics
  • Signal Transduction / physiology
  • Transcription Factor 7-Like 2 Protein / genetics
  • Transcription Factor 7-Like 2 Protein / physiology*
  • Wnt Signaling Pathway / genetics
  • Wnt Signaling Pathway / physiology*
  • beta Catenin* / metabolism

Substances

  • Bone Morphogenetic Proteins
  • Tcf7l2 protein, mouse
  • Transcription Factor 7-Like 2 Protein
  • beta Catenin