Water-soluble L-cysteine-coated FePt nanoparticles as dual MRI/CT imaging contrast agent for glioma

Int J Nanomedicine. 2015 Mar 23:10:2325-33. doi: 10.2147/IJN.S75174. eCollection 2015.

Abstract

Nanoparticles (NPs) are advantageous for the delivery of diagnosis agents to brain tumors. In this study, we attempted to develop an L-cysteine coated FePt (FePt-Cys) NP as MRI/CT imaging contrast agent for the diagnosis of malignant gliomas. FePt-Cys NPs were synthesized through a co-reduction route, which was characterized by transmission electron microscopy, high-resolution transmission electron microscopy, powder X-ray diffraction, Fourier transform infrared spectroscopy, and dynamic light scattering. The MRI and CT imaging ability of FePt-Cys NPs was evaluated using different gliomas cells (C6, SGH44, U251) as the model. Furthermore, the biocompatibility of the as-synthesized FePt-Cys NPs was evaluated using three different cell lines (ECV304, L929, and HEK293) as the model. The results showed that FePt-Cys NPs displayed excellent biocompatibility and good MRI/CT imaging ability, thereby indicating promising potential as a dual MRI/CT contrast agent for the diagnosis of brain malignant gliomas.

Keywords: CT; MRI; glioma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Contrast Media / chemistry*
  • Contrast Media / metabolism
  • Cysteine / chemistry*
  • Cysteine / metabolism
  • Glioma / metabolism*
  • HEK293 Cells
  • Humans
  • Iron / chemistry*
  • Iron / metabolism
  • Magnetic Resonance Imaging / methods*
  • Metal Nanoparticles / chemistry*
  • Platinum / chemistry*
  • Platinum / metabolism
  • Tomography, X-Ray Computed / methods

Substances

  • Contrast Media
  • Platinum
  • Iron
  • Cysteine