Epidemiological survey on Cryptosporidium in an Equine Perinatology Unit

R Galuppi; S Piva; C Castagnetti; E Iacono; S Tanel; F Pallaver; M L Fioravanti; R G Zanoni; M P Tampieri; M Caffara

doi:10.1016/j.vetpar.2015.03.021

Epidemiological survey on Cryptosporidium in an Equine Perinatology Unit

Vet Parasitol. 2015 May 30;210(1-2):10-8. doi: 10.1016/j.vetpar.2015.03.021. Epub 2015 Mar 30.

Authors

R Galuppi¹, S Piva², C Castagnetti², E Iacono², S Tanel², F Pallaver², M L Fioravanti², R G Zanoni², M P Tampieri², M Caffara²

Affiliations

¹ Department of Veterinary Medical Sciences, Alma Mater Studiorum - Bologna University, Via Tolara di Sopra, 50, 40064 Ozzano Emilia, BO, Italy. Electronic address: roberta.galuppi@unibo.it.
² Department of Veterinary Medical Sciences, Alma Mater Studiorum - Bologna University, Via Tolara di Sopra, 50, 40064 Ozzano Emilia, BO, Italy.

PMID: 25868850
DOI: 10.1016/j.vetpar.2015.03.021

Abstract

The present study aims to evaluate the prevalence, pattern of spread and risk factors for the transmission of cryptosporidiosis in foals and mares hospitalized in a University Equine Perinatology Unit, where a new subtype family of Cryptosporidium horse genotype was described by Caffara et al. (2013). Mares (36) and foals (37) hospitalized during the 2012 foaling season were included. Multiple sampling from each animal was performed (a total of 305 stool samples were collected). One hundred and eleven environmental samples (gauze swabs) were also collected before and after the breeding season. Fourteen foals were found positive for Cryptosporidium spp. by PCR in at least one sample; a total of 35 foal stool specimens were confirmed for the presence of the protozoa. Instead none of the stool specimens from mares were found positive. PCR-RFLP analysis shows Cryptosporidium parvum in 5 stool samples and Cryptosporidium horse genotype in 21. In 9 specimens, from 4 different foals, the profile was suggestive for a mixed infection. The subtyping at gp60 locus showed 2 strains as members of the subtype family IId and six of the subfamily IIa of C. parvum. Twenty isolates were identified as Cryptosporidium horse genotype subtype VIaA15G4. Five gauze swabs collected from the walls of the boxes where the animals were hosted out of 111 environmental samples examined were PCR positive for Cryptosporidium spp. Cryptosporidium parvum was detected in one sample collected before the foaling season, while Cryptosporidium horse genotype profile was observed in 4 wall samples collected at the end of the 2012 foaling season. The prevalence observed in foals (37.8%) was higher than that reported in other studies. These features and the diffusion of the same genotype point out as the EPU, where critically ill foals are hospitalized, can support the spread of cryptosporidiosis. Therefore, the manual tasks and the activities carried out in these facilities are of great importance, as they might favor the diffusion of the infection.

Keywords: Cryptosporidium; Epidemiology; Equine Perinatology Unit; Foals.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Newborn
Cryptosporidiosis / epidemiology
Cryptosporidiosis / parasitology*
Cryptosporidium / classification
Cryptosporidium / genetics*
Cryptosporidium / isolation & purification
Feces / parasitology
Female
Gene Expression Profiling / veterinary
Genotype
Horse Diseases / epidemiology
Horse Diseases / parasitology*
Horses
Prevalence