Increased LDL electronegativity in chronic kidney disease disrupts calcium homeostasis resulting in cardiac dysfunction

J Mol Cell Cardiol. 2015 Jul:84:36-44. doi: 10.1016/j.yjmcc.2015.03.016. Epub 2015 Apr 11.

Abstract

Chronic kidney disease (CKD), an independent risk factor for cardiovascular disease, is associated with abnormal lipoprotein metabolism. We examined whether electronegative low-density lipoprotein (LDL) is mechanistically linked to cardiac dysfunction in patients with early CKD. We compared echocardiographic parameters between patients with stage 2 CKD (n = 88) and normal controls (n = 89) and found that impaired relaxation was more common in CKD patients. Reduction in estimated glomerular filtration rate was an independent predictor of left ventricular relaxation dysfunction. We then examined cardiac function in a rat model of early CKD induced by unilateral nephrectomy (UNx) by analyzing pressure-volume loop data. The time constant of isovolumic pressure decay was longer and the maximal velocity of pressure fall was slower in UNx rats than in controls. When we investigated the mechanisms underlying relaxation dysfunction, we found that LDL from CKD patients and UNx rats was more electronegative than LDL from their respective controls and that LDL from UNx rats induced intracellular calcium overload in H9c2 cardiomyocytes in vitro. Furthermore, chronic administration of electronegative LDL, which signals through lectin-like oxidized LDL receptor-1 (LOX-1), induced relaxation dysfunction in wild-type but not LOX-1(-/-) mice. In in vitro and in vivo experiments, impaired cardiac relaxation was associated with increased calcium transient resulting from nitric oxide (NO)-dependent nitrosylation of SERCA2a due to increases in inducible NO synthase expression and endothelial NO synthase uncoupling. In conclusion, LDL becomes more electronegative in early CKD. This change disrupts SERCA2a-regulated calcium homeostasis, which may be the mechanism underlying cardiorenal syndrome.

Keywords: Cardiorenal syndrome; Lipoproteins; SERCA2a; Unilateral nephrectomy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Calcium / metabolism*
  • Case-Control Studies
  • Demography
  • Female
  • Fibrosis
  • Heart
  • Homeostasis*
  • Humans
  • Lipoproteins, LDL / metabolism*
  • Male
  • Mice, Inbred C57BL
  • Models, Biological
  • Myocytes, Cardiac / metabolism
  • Nephrectomy
  • Nitric Oxide Synthase Type II / metabolism
  • Nitrosation
  • Rats, Sprague-Dawley
  • Receptors, Oxidized LDL / metabolism
  • Renal Insufficiency, Chronic / diagnostic imaging
  • Renal Insufficiency, Chronic / metabolism*
  • Renal Insufficiency, Chronic / physiopathology*
  • Renin-Angiotensin System
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases / metabolism
  • Ultrasonography
  • Up-Regulation
  • Vasodilation
  • tau Proteins / metabolism

Substances

  • Lipoproteins, LDL
  • Receptors, Oxidized LDL
  • oxidized low density lipoprotein
  • tau Proteins
  • Nitric Oxide Synthase Type II
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases
  • Calcium