Cellular inhibitor of apoptosis proteins prevent clearance of hepatitis B virus

Proc Natl Acad Sci U S A. 2015 May 5;112(18):5797-802. doi: 10.1073/pnas.1502390112. Epub 2015 Apr 20.

Abstract

Hepatitis B virus (HBV) infection can result in a spectrum of outcomes from immune-mediated control to disease progression, cirrhosis, and liver cancer. The host molecular pathways that influence and contribute to these outcomes need to be defined. Using an immunocompetent mouse model of chronic HBV infection, we identified some of the host cellular and molecular factors that impact on infection outcomes. Here, we show that cellular inhibitor of apoptosis proteins (cIAPs) attenuate TNF signaling during hepatitis B infection, and they restrict the death of infected hepatocytes, thus allowing viral persistence. Animals with a liver-specific cIAP1 and total cIAP2 deficiency efficiently control HBV infection compared with WT mice. This phenotype was partly recapitulated in mice that were deficient in cIAP2 alone. These results indicate that antagonizing the function of cIAPs may promote the clearance of HBV infection.

Keywords: TNF; cIAP1; cIAP2; cellular inhibitor of apoptosis proteins; hepatitis B virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Baculoviral IAP Repeat-Containing 3 Protein
  • CD4 Antigens / metabolism
  • CD8 Antigens / metabolism
  • Cytokines / metabolism
  • DNA, Viral / genetics
  • Disease Models, Animal
  • Genotype
  • Hepatitis B / metabolism*
  • Hepatitis B virus*
  • Hepatocytes / metabolism
  • Hepatocytes / virology
  • Immunophenotyping
  • Immunosuppression Therapy
  • Inhibitor of Apoptosis Proteins / metabolism*
  • Interferon-gamma / metabolism
  • Liver / metabolism
  • Liver Neoplasms / metabolism
  • Liver Neoplasms / virology
  • Mice
  • Mice, Inbred C57BL
  • Phenotype
  • Signal Transduction
  • Tumor Necrosis Factor-alpha / metabolism
  • Ubiquitin-Protein Ligases / metabolism*

Substances

  • CD4 Antigens
  • CD8 Antigens
  • Cytokines
  • DNA, Viral
  • Inhibitor of Apoptosis Proteins
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma
  • Baculoviral IAP Repeat-Containing 3 Protein
  • Birc2 protein, mouse
  • Birc3 protein, mouse
  • Ubiquitin-Protein Ligases