Immortalization of epithelial cells in oral carcinogenesis as revealed by genome-wide array comparative genomic hybridization: A meta-analysis

Head Neck. 2016 Apr:38 Suppl 1:E783-97. doi: 10.1002/hed.24102. Epub 2015 Jul 15.

Abstract

Background: This purpose of this meta-analysis study was to identify the most frequent and potentially significant copy number alteration (CNA) in oral carcinogenesis.

Methods: Seven oral squamous cell carcinoma (OSCC)-related publications, corresponding to 312 samples, were identified for this meta-analysis. The data were analyzed in a 4-step process that included the genome assembly coordination of multiple platforms, assignment of chromosomal position anchors, calling gains and losses, and functional annotation analysis.

Results: Gains were more frequent than losses in the entire dataset. High-frequency gains were identified in chromosomes 5p, 14q, 11q, 7p, 17q, 20q, 8q, and 3q, whereas high-frequency losses were identified in chromosomes 3p, 8p, 6p, 18q, and 4q. Ingenuity pathway analysis showed that the top biological function was associated with immortalization of the epithelial cells (p = 1.93E-04).

Conclusion: This study has identified multiple recurrent CNAs that are involved in various biological annotations associated with oral carcinogenesis. © 2015 Wiley Periodicals, Inc. Head Neck 38: E783-E797, 2016.

Keywords: array comparative genomic hybridization (CGH); copy number alteration; meta-analysis; oral carcinogenesis; oral squamous cell carcinoma (OSCC).

Publication types

  • Meta-Analysis

MeSH terms

  • Carcinogenesis*
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / pathology
  • Chromosome Aberrations
  • Comparative Genomic Hybridization
  • DNA Copy Number Variations*
  • Epithelial Cells / pathology*
  • Humans
  • Mouth Neoplasms / genetics*
  • Mouth Neoplasms / pathology