Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma

Ali A Albarhani; Fiona Collier; Ronda F Greaves; Anne-Louise Ponsonby; Katrina J Allen; Peter J Vuillermin; Peter Roche; Michael W Clarke; BIS Steering Committee

doi:10.1016/j.clinbiochem.2015.04.014

Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma

Clin Biochem. 2015 Nov;48(16-17):1105-12. doi: 10.1016/j.clinbiochem.2015.04.014. Epub 2015 Apr 25.

Authors

Ali A Albarhani¹, Fiona Collier², Ronda F Greaves³, Anne-Louise Ponsonby⁴, Katrina J Allen⁴, Peter J Vuillermin⁵, Peter Roche⁶, Michael W Clarke⁷; BIS Steering Committee

Collaborators

BIS Steering Committee:
Katie Allen, David Burgner, John Carlin, Terry Dwyer, Anne-Louise Ponsonby, Sarath Ranganathan, Richard Saffery, Mimi Tang, Peter Vuillermin

Affiliations

¹ School of Medical Sciences, RMIT University, Victoria, Australia; Security Forces Hospital, Dammam, Saudi Arabia.
² Deakin University, Victoria, Australia.
³ School of Medical Sciences, RMIT University, Victoria, Australia; Murdoch Children's Research Institute, Melbourne, Australia.
⁴ Murdoch Children's Research Institute, Melbourne, Australia; Department of Paediatrics, University of Melbourne, Victoria, Australia.
⁵ Deakin University, Victoria, Australia; Child Health Research Unit, Barwon Health, Victoria Australia.
⁶ School of Medical Sciences, RMIT University, Victoria, Australia.
⁷ University of Western Australia, Western Australia, Australia. Electronic address: michael.clarke@uwa.edu.au.

PMID: 25920890
DOI: 10.1016/j.clinbiochem.2015.04.014

Abstract

Objectives: In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for vitamin D, A and E analysis compared to UCB serum samples.

Design & methods: Twenty UCB matched samples of diluted plasma and serum were collected. The samples were analysed by two liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods on two separate occasions.

Results: The results of 25(OH)D3 obtained by the two laboratories demonstrated close agreement with a mean difference of 0.14nmol/L [95% confidence interval (95% CI), -6.8 to 7.1]. Both methods demonstrate close agreement for 25(OH)D3 in UCB serum versus diluted UCB plasma; mean difference 2.2nmol/L [95% CI, -9.5 to 13.9] and 4.1nmol/L [95% CI, -14.5 to 6.1] for the results from Lab A and Lab B, respectively. Vitamin A was quantified by Lab A in UCB serum and diluted UCB plasma; mean difference 0.07μmol/L [95% CI, -0.41 to 0.28]. Results of 25(OH)D3 epimer and vitamin E in the diluted UCB plasma were below the limit of quantification, and could not be compared with UCB serum.

Conclusions: Diluted UCB plasma can be used for the quantification of retinol and 25(OH)D3 by LC-MS/MS. By contrast, quantification of 25(OH)D3 epimer and vitamin E in diluted UCB plasma is not supported by this study due to limitations in analytical sensitivity.

Keywords: Cholecalciferol; Mass spectrometry; Neonates; Retinol; Umbilical cord blood diluted plasma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid / methods
Fetal Blood / chemistry*
Humans
Plasma / chemistry*
Tandem Mass Spectrometry / methods
Vitamin A / blood*
Vitamin D / blood*
Vitamin E / blood

Substances

Vitamin A
Vitamin D
Vitamin E