Modifications of collagen-based biomaterials with immobilized growth factors or peptides

Yun Xiao; Lewis A Reis; Yimu Zhao; Milica Radisic

doi:10.1016/j.ymeth.2015.04.025

Modifications of collagen-based biomaterials with immobilized growth factors or peptides

Methods. 2015 Aug:84:44-52. doi: 10.1016/j.ymeth.2015.04.025. Epub 2015 May 4.

Authors

Yun Xiao¹, Lewis A Reis², Yimu Zhao³, Milica Radisic⁴

Affiliations

¹ Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
² Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
³ Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada.
⁴ Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada; Toronto General Research Institute, University Health Network and IBBME, University of Toronto, Toronto, ON, Canada. Electronic address: m.radisic@utoronto.ca.

PMID: 25952946
DOI: 10.1016/j.ymeth.2015.04.025

Abstract

In order to provide an instructive microenvironment to facilitate cellular behaviors and tissue regeneration, biomaterials can be modified by immobilizing growth factors or peptides. We describe here our procedure for modification of collagen-based biomaterials, both porous scaffolds and hydrogel systems, with growth factors or peptides by covalent immobilization. Characterizations of the modified biomaterials (immobilization efficiency, release profile, morphology, mechanical strength, and rheology) and in vitro testing with cells are also discussed.

Keywords: Basic fibroblast growth factor; Chitosan; Collagen; Growth factor; Hydrogel; Peptide; Porous scaffold; QHREDGS; Vascular endothelial growth factor.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Biocompatible Materials / chemistry*
Cell Culture Techniques / methods
Chitosan / chemistry
Collagen / chemistry*
Humans
Hydrogels / chemistry
Immobilized Proteins / administration & dosage
Immobilized Proteins / chemistry
Immobilized Proteins / pharmacokinetics
Intercellular Signaling Peptides and Proteins / administration & dosage
Intercellular Signaling Peptides and Proteins / chemistry
Intercellular Signaling Peptides and Proteins / pharmacokinetics
Materials Testing
Porosity
Regenerative Medicine / methods
Rheology
Tensile Strength
Tissue Engineering / methods*

Substances

Biocompatible Materials
Hydrogels
Immobilized Proteins
Intercellular Signaling Peptides and Proteins
Collagen
Chitosan

Abstract

Publication types

MeSH terms

Substances

Grants and funding