The tRNA Splicing Endonuclease Complex Cleaves the Mitochondria-localized CBP1 mRNA

Tatsuhisa Tsuboi; Reina Yamazaki; Risa Nobuta; Ken Ikeuchi; Shiho Makino; Ayumi Ohtaki; Yutaka Suzuki; Tohru Yoshihisa; Christopher Trotta; Toshifumi Inada

doi:10.1074/jbc.M114.634592

The tRNA Splicing Endonuclease Complex Cleaves the Mitochondria-localized CBP1 mRNA

J Biol Chem. 2015 Jun 26;290(26):16021-30. doi: 10.1074/jbc.M114.634592. Epub 2015 May 13.

Authors

Affiliations

¹ From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
² the Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
³ the Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan, and.
⁴ the PTC Therapeutics, South Plainfield, New Jersey 07080.
⁵ From the Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan, tinada@m.tohoku.ac.jp.

Abstract

The tRNA splicing endonuclease (Sen) complex is located on the mitochondrial outer membrane and splices precursor tRNAs in Saccharomyces cerevisiae. Here, we demonstrate that the Sen complex cleaves the mitochondria-localized mRNA encoding Cbp1 (cytochrome b mRNA processing 1). Endonucleolytic cleavage of this mRNA required two cis-elements: the mitochondrial targeting signal and the stem-loop 652-726-nt region. Mitochondrial localization of the Sen complex was required for cleavage of the CBP1 mRNA, and the Sen complex cleaved this mRNA directly in vitro. We propose that the Sen complex cleaves the CBP1 mRNA, which is co-translationally localized to mitochondria via its mitochondrial targeting signal.

Keywords: endonuclease; mRNA decay; mRNA decay, endonuclease; mRNA localization; mitochondria; quality control; tRNA splicing; transfer RNA (tRNA); translation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Endoribonucleases / genetics
Endoribonucleases / metabolism*
Mitochondria / genetics
Mitochondria / metabolism*
Mitochondrial Proteins / chemistry
Mitochondrial Proteins / genetics*
Mitochondrial Proteins / metabolism
Molecular Sequence Data
Nucleic Acid Conformation
RNA Splicing
RNA, Messenger / chemistry
RNA, Messenger / genetics*
RNA, Messenger / metabolism
RNA-Binding Proteins / chemistry
RNA-Binding Proteins / genetics*
RNA-Binding Proteins / metabolism
Saccharomyces cerevisiae / chemistry
Saccharomyces cerevisiae / enzymology*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / chemistry
Saccharomyces cerevisiae Proteins / genetics*
Saccharomyces cerevisiae Proteins / metabolism

Substances

Mitochondrial Proteins
RNA, Messenger
RNA-Binding Proteins
Saccharomyces cerevisiae Proteins
cbp1 protein, S cerevisiae
Endoribonucleases
splicing endonuclease