Rapid Detection of KPC, NDM, and OXA-48-Like Carbapenemases by Real-Time PCR from Rectal Swab Surveillance Samples

Tracy D Lee; Kathleen Adie; Alan McNabb; Dale Purych; Kulvinder Mannan; Robert Azana; Corrinne Ng; Patrick Tang; Linda M N Hoang

doi:10.1128/JCM.01237-15

Rapid Detection of KPC, NDM, and OXA-48-Like Carbapenemases by Real-Time PCR from Rectal Swab Surveillance Samples

J Clin Microbiol. 2015 Aug;53(8):2731-3. doi: 10.1128/JCM.01237-15. Epub 2015 May 27.

Authors

Tracy D Lee¹, Kathleen Adie¹, Alan McNabb¹, Dale Purych², Kulvinder Mannan³, Robert Azana¹, Corrinne Ng¹, Patrick Tang⁴, Linda M N Hoang⁵

Affiliations

¹ Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, PHSA Laboratories, Vancouver, British Columbia, Canada.
² Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada Department of Laboratory Medicine and Pathology, Fraser Health, Surrey, British Columbia, Canada.
³ Department of Laboratory Medicine and Pathology, Fraser Health, Surrey, British Columbia, Canada.
⁴ Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, PHSA Laboratories, Vancouver, British Columbia, Canada Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
⁵ Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, PHSA Laboratories, Vancouver, British Columbia, Canada Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada linda.hoang@bccdc.ca.

Abstract

We describe a multiplex real-time PCR assay for use on the ABI 7500 Fast TaqMan platform to detect all currently described Klebsiella pneumoniae carbapenemases (KPC), New Delhi metallo-β-lactamases (NDM), and the OXA-48-like family of carbapenemases from bacterial culture lysates or sample enrichment broth lysates.

Publication types

Evaluation Study

MeSH terms

Bacterial Proteins / analysis*
Bacterial Proteins / genetics
Epidemiological Monitoring*
Gram-Negative Bacteria / enzymology*
Gram-Negative Bacteria / genetics
Humans
Multiplex Polymerase Chain Reaction / methods*
Real-Time Polymerase Chain Reaction / methods*
Rectum / microbiology*
beta-Lactamases / analysis*
beta-Lactamases / genetics

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase