A field experiment with two broomcorn millet varieties Longmi 8 (strong drought-resistant variety) and Jinmi 4 (drought-sensitive variety) was conducted to compare their differences in growth, field microclimate and photosynthetic capacity from anthesis to maturity under different fertility conditions. The results showed that, fertilization decreased canopy temperature, air temperature, soil temperature, illumination, but improved the relative humidity among broomcorn millet plants compared with the non-fertilization treatment. With an increase of the fertilizer level, the plant height, SPAD, LAI, net photosynthetic rate, transpiration rate, stomatal conductance, intercellular CO2 concentration in broomcorn millet showed an increasing trend, which of the high fertilization treatment were 9.2%, 15.1%, 56.6%, 17.8%, 24.6%, 14.2%, 29.7% higher than those of non-fertilization treatment, respectively. Compared with Jinmi 4, Longmi 8 showed a cold wet characteristic, with lower canopy temperature, air temperature, soil temperature; illumination, and higher plant height, LAI, SPAD and relative humidity during grain filling. Moreover, each photosynthetic index of Longmi 8 slowly decreased and extended the period of leaf photosynthetic function so as to accumulate more photosynthetic products.