Label-free fluorescence light-up detection of T4 polynucleotide kinase activity using the split-to-intact G-quadruplex strategy by ligation-triggered and toehold-mediated strand displacement release

Lu Zhou; Xiaoqiang Shen; Na Sun; Kewei Wang; Yuanyuan Zhang; Renjun Pei

doi:10.1039/c5an01032b

Label-free fluorescence light-up detection of T4 polynucleotide kinase activity using the split-to-intact G-quadruplex strategy by ligation-triggered and toehold-mediated strand displacement release

Analyst. 2015 Aug 21;140(16):5450-3. doi: 10.1039/c5an01032b.

Authors

Lu Zhou¹, Xiaoqiang Shen, Na Sun, Kewei Wang, Yuanyuan Zhang, Renjun Pei

Affiliation

¹ Key Laboratory of Nano-Bio Interfacce, Division of Nanobiomedicine, Suzhou Insitute of Nano-Tech and Nano-Bionics, Chinese Academy of Science, Suzhou, 215123, China. rjpei2011@sinano.ac.cn.

PMID: 26098904
DOI: 10.1039/c5an01032b

Abstract

A label-free, fluorescence light-up detection method for T4 polynucleotide kinase activity has been developed using the split-to-intact G-quadruplex strategy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / methods*
Breast Neoplasms / enzymology
Cells, Cultured
DNA / chemistry*
Erythrocytes / enzymology
Female
Fluorescence
G-Quadruplexes*
Humans
Light*
Phosphorylation
Polynucleotide 5'-Hydroxyl-Kinase / metabolism*
Precursor Cell Lymphoblastic Leukemia-Lymphoma / enzymology
Spectrometry, Fluorescence

Substances

DNA
Polynucleotide 5'-Hydroxyl-Kinase