The GATA transcription factor GtaC regulates early developmental gene expression dynamics in Dictyostelium

Nat Commun. 2015 Jul 6:6:7551. doi: 10.1038/ncomms8551.

Abstract

In many systems, including the social amoeba Dictyostelium discoideum, development is often marked by dynamic morphological and transcriptional changes orchestrated by key transcription factors. However, efforts to examine sequential genome-wide changes of gene regulation in developmental processes have been fairly limited. Here we report the developmental regulatory dynamics of GtaC, a GATA-type zinc-finger transcription factor, through the analyses of serial ChIP- and RNA-sequencing data. GtaC is essential for developmental progression, decoding extracellular cAMP pulses during early development and may play a role in mediating cell-type differentiation at later stages. We find that GtaC exhibits temporally distinctive DNA-binding patterns concordant with each developmental stage. We identify direct GtaC targets and observe cotemporaneous GtaC-binding and developmental expression regulation. Our results suggest that GtaC regulates multiple physiological processes as Dictyostelium transitions from a group of unicellular amoebae to an integrated multicellular organism.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / metabolism
  • Dictyostelium / genetics
  • Dictyostelium / metabolism*
  • Gene Expression Regulation, Developmental / physiology*
  • Protein Binding
  • Protozoan Proteins / genetics
  • Protozoan Proteins / metabolism*
  • RNA / genetics
  • RNA / metabolism
  • RNA, Protozoan / genetics
  • RNA, Protozoan / metabolism
  • Transcriptome

Substances

  • Protozoan Proteins
  • RNA, Protozoan
  • RNA
  • DNA

Associated data

  • GEO/GSE63151