Comparative Genomic Analysis of Asian Haemorrhagic Septicaemia-Associated Strains of Pasteurella multocida Identifies More than 90 Haemorrhagic Septicaemia-Specific Genes

PLoS One. 2015 Jul 7;10(7):e0130296. doi: 10.1371/journal.pone.0130296. eCollection 2015.

Abstract

Pasteurella multocida is the primary causative agent of a range of economically important diseases in animals, including haemorrhagic septicaemia (HS), a rapidly fatal disease of ungulates. There is limited information available on the diversity of P. multocida strains that cause HS. Therefore, we determined draft genome sequences of ten disease-causing isolates and two vaccine strains and compared these genomes using a range of bioinformatic analyses. The draft genomes of the 12 HS strains were between 2,298,035 and 2,410,300 bp in length. Comparison of these genomes with the North American HS strain, M1404, and other available P. multocida genomes (Pm70, 3480, 36950 and HN06) identified a core set of 1,824 genes. A set of 96 genes was present in all HS isolates and vaccine strains examined in this study, but absent from Pm70, 3480, 36950 and HN06. Moreover, 59 genes were shared only by the Asian B:2 strains. In two Pakistani isolates, genes with high similarity to genes in the integrative and conjugative element, ICEPmu1 from strain 36950 were identified along with a range of other antimicrobial resistance genes. Phylogenetic analysis indicated that the HS strains formed clades based on their country of isolation. Future analysis of the 96 genes unique to the HS isolates will aid the identification of HS-specific virulence attributes and facilitate the development of disease-specific diagnostic tests.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Asia
  • Bacterial Proteins / genetics
  • Base Sequence
  • Cattle
  • Cattle Diseases / genetics*
  • Cattle Diseases / microbiology
  • Cattle Diseases / pathology
  • Comparative Genomic Hybridization
  • DNA, Bacterial / analysis
  • Drug Resistance, Bacterial / genetics
  • Genome, Bacterial*
  • Hemorrhagic Septicemia / genetics*
  • Hemorrhagic Septicemia / microbiology
  • Hemorrhagic Septicemia / pathology
  • High-Throughput Nucleotide Sequencing
  • Lipopolysaccharides / biosynthesis
  • Molecular Sequence Data
  • Pakistan
  • Pasteurella multocida / classification
  • Pasteurella multocida / genetics*
  • Pasteurella multocida / isolation & purification
  • Phylogeny
  • Polymorphism, Single Nucleotide
  • Sequence Analysis, DNA

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Lipopolysaccharides

Grants and funding

Funding was provided by the Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Grant number CE0562063; Australian Research Council, http://www.arc.gov.au/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.