Antibodies to VP1 of swine pasivirus in humans without evidence of transmission from a pig source

J Clin Virol. 2015 Aug:69:36-9. doi: 10.1016/j.jcv.2015.05.026. Epub 2015 Jun 3.

Abstract

Background: Swine pasivirus (SPaV1) is a recently described enteric virus close to human parechoviruses and highly prevalent in pigs. Antibodies to Escherichia coli-expressed VP1 of SpaV1 have been found in a majority of humans in China.

Objectives: The objectives were to estimate the antibody prevalence in a European country, to test if exposure to the virus was linked to pig products and if this exposure was a risk factor for the development of diabetes type 1.

Study design: An ELISA test was developed and used to screen 842 healthy subjects with known exposure to pig products, 39 patients with diabetes type 1 and 20 controls.

Results: We identified a high seroprevalence (15.6%) reacting to VP1 of SPaV1 among healthy human subjects. Analysis of risk factors argues against cross-species transmission from pigs as the source of infection. Data also indicate that the presence of SPaV1 VP1-binding antibodies is not associated with diabetes type 1 in humans.

Conclusion: Our results suggest that the seroreactivity frequently found in humans against SpaV1 is due to cross-reactivity with related antigen, perhaps a picornavirus, and that SpaV1 is not a zoonotic virus.

Keywords: Human; Parechovirus; Pasivirus; Picornaviridae; Swine; Zoonosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Antibodies, Viral / blood*
  • Child
  • Child, Preschool
  • China
  • Cross Reactions
  • Diabetes Mellitus, Type 1 / blood
  • Diabetes Mellitus, Type 1 / etiology
  • Diabetes Mellitus, Type 1 / immunology
  • Diabetes Mellitus, Type 1 / virology
  • Enzyme-Linked Immunosorbent Assay
  • Europe / epidemiology
  • Female
  • Humans
  • Male
  • Middle Aged
  • Picornaviridae / immunology*
  • Picornaviridae Infections / epidemiology
  • Picornaviridae Infections / transmission
  • Picornaviridae Infections / veterinary
  • Risk Factors
  • Seroepidemiologic Studies
  • Swine
  • Swine Diseases / virology
  • Viral Structural Proteins / immunology*
  • Young Adult

Substances

  • Antibodies, Viral
  • Viral Structural Proteins