First Complete Genome Sequence of Clostridium sporogenes DSM 795T, a Nontoxigenic Surrogate for Clostridium botulinum, Determined Using PacBio Single-Molecule Real-Time Technology

Kazuma Nakano; Yasunobu Terabayashi; Akino Shiroma; Makiko Shimoji; Hinako Tamotsu; Noriko Ashimine; Shun Ohki; Misuzu Shinzato; Kuniko Teruya; Kazuhito Satou; Takashi Hirano

doi:10.1128/genomeA.00832-15

First Complete Genome Sequence of Clostridium sporogenes DSM 795T, a Nontoxigenic Surrogate for Clostridium botulinum, Determined Using PacBio Single-Molecule Real-Time Technology

Genome Announc. 2015 Jul 30;3(4):e00832-15. doi: 10.1128/genomeA.00832-15.

Affiliations

¹ Okinawa Institute of Advanced Sciences, Uruma, Okinawa, Japan nakano@oias.or.jp.
² Okinawa Institute of Advanced Sciences, Uruma, Okinawa, Japan.

Abstract

The first complete genome sequence of Clostridium sporogenes DSM 795(T), a nontoxigenic surrogate for Clostridium botulinum, was determined in a single contig using the PacBio single-molecule real-time technology. The genome (4,142,990 bp; G+C content, 27.98%) included 86 sets of >1,000-bp identical sequence pairs and 380 tandem repeats.