The reproductive potentials of strains Yersinia pseudotuberculosis serovars I, II, III, IV and V cultivated at different temperatures on media containing rhamnose, raffinose and sodium succinate were examined comparatively. It was established that the growth of serovars I, II and III was activated by sodium succinate at temperatures 4 degrees and 25 degrees C, while serovars IV and V showed lower growth rate regardless of the substrate used. At 37 degrees C D-raffinose activated the growth of the cells of serovars IV and V. The studies on the effect of different substrates on the growth of Yersinia pseudotuberculosis in a wide temperature range acquire great importance for the investigating of the process of infection, revealing some aspects of the microorganism-macroorganism relationship.