Abstract
A series of structure based drug design hypotheses and focused screening efforts led to the identification of tetrahydropyrrolo-diazepenones with striking potency against ERK2 kinase. The role of fluorination in mitigating microsomal clearance was systematically explored. Ultimately, it was found that fluorination of a cyclopentanol substructure provided significant improvement in both potency and human metabolic stability.
Keywords:
ATP binding cleft; ERK2 kinase; Extraction ratio; Lower-hinge lysine; Structure based drug design.
Copyright © 2015 Elsevier Ltd. All rights reserved.
MeSH terms
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Azepines / chemistry
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Azepines / pharmacology*
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Crystallography, X-Ray
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Dose-Response Relationship, Drug
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Heterocyclic Compounds, 2-Ring / chemistry
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Heterocyclic Compounds, 2-Ring / pharmacology*
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Humans
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Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors*
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Mitogen-Activated Protein Kinase 1 / metabolism
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Models, Molecular
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Molecular Structure
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Protein Kinase Inhibitors / chemical synthesis
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Protein Kinase Inhibitors / chemistry
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Protein Kinase Inhibitors / pharmacology*
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Pyrroles / chemistry
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Pyrroles / pharmacology*
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Structure-Activity Relationship
Substances
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Azepines
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Heterocyclic Compounds, 2-Ring
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Protein Kinase Inhibitors
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Pyrroles
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Mitogen-Activated Protein Kinase 1