Purification of Thymocyte and T Cell Subsets

Methods Mol Biol. 2016:1323:87-97. doi: 10.1007/978-1-4939-2809-5_7.

Abstract

Many analytical or cell culture procedures require homogeneous starting cell populations that cannot be obtained directly from organ dissection. Here, we describe two enrichment procedures to achieve this goal and discuss their respective advantages in specific experimental contexts. Notes in this chapter include some tips on how to determine the appropriate level of purity (see Note 1 ).

MeSH terms

  • Flow Cytometry / methods
  • Immunomagnetic Separation / methods*
  • Immunophenotyping
  • T-Lymphocyte Subsets / cytology*
  • T-Lymphocyte Subsets / metabolism*
  • Thymocytes / cytology*
  • Thymocytes / metabolism*