Abstract
In vitro culture is an important complement, or substitute, to in vivo approaches in order to study T cell effector differentiation. Here, we describe culture conditions that generate specific effector cell types by exposing naïve T cells to appropriate cytokine signals.
MeSH terms
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Animals
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Antigen-Presenting Cells / cytology
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Antigen-Presenting Cells / metabolism
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Cell Differentiation*
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Cell Separation
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Dendritic Cells / cytology
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Dendritic Cells / metabolism
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Flow Cytometry
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Fluorescent Antibody Technique
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Immunophenotyping
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In Vitro Techniques
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Mice
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Spleen / cytology
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T-Lymphocyte Subsets / cytology*
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T-Lymphocyte Subsets / metabolism*