Interleukin-18, interleukin-12B and interferon-γ gene polymorphisms in Brazilian patients with rheumatoid arthritis: a pilot study

Tissue Antigens. 2015 Oct;86(4):276-8. doi: 10.1111/tan.12645. Epub 2015 Aug 25.

Abstract

Polymorphisms in interleukin (IL)-18, IL-12 and interferon (IFN)-γ genes are associated with different levels of cytokines expression and have been associated with rheumatoid arthritis (RA). IL-18 +105 A/C, IL-12B +1188 A/C and IFN-γ +874 T/A polymorphisms were analyzed by restriction fragment length polymorphism-polymerase chain reaction (PCR) and amplification refractory mutation system PCR from 90 RA patients and 186 healthy individuals. There were significant differences to IL-18 +105 A/C polymorphism between the RA and control groups (odds ratio = 3.77; P < 0.0001). Individual carriers of the variant allele C had a 3.77-fold increased risk of for RA (P = 0.0032). No association was observed for IL-12B and IFN-γ polymorphisms. Our finds suggest a possible role for IL-18 polymorphism in the RA susceptibility in studied population.

Keywords: arthritis; cytokines; polymorphism; rheumatoid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Alleles
  • Arthritis, Rheumatoid / genetics*
  • Arthritis, Rheumatoid / immunology
  • Arthritis, Rheumatoid / pathology
  • Brazil
  • Case-Control Studies
  • Female
  • Gene Expression
  • Gene Frequency
  • Genetic Predisposition to Disease*
  • Humans
  • Interferon-gamma / genetics*
  • Interferon-gamma / immunology
  • Interleukin-12 Subunit p40 / genetics*
  • Interleukin-12 Subunit p40 / immunology
  • Interleukin-18 / genetics*
  • Interleukin-18 / immunology
  • Male
  • Middle Aged
  • Pilot Projects
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single Nucleotide*
  • Risk

Substances

  • IL12B protein, human
  • IL18 protein, human
  • Interleukin-12 Subunit p40
  • Interleukin-18
  • Interferon-gamma