Haustorium Formation in Medicago truncatula Roots Infected by Phytophthora palmivora Does Not Involve the Common Endosymbiotic Program Shared by Arbuscular Mycorrhizal Fungi and Rhizobia

Mol Plant Microbe Interact. 2015 Dec;28(12):1271-80. doi: 10.1094/MPMI-06-15-0130-R. Epub 2015 Oct 29.

Abstract

In biotrophic plant-microbe interactions, microbes infect living plant cells, in which they are hosted in a novel membrane compartment, the host-microbe interface. To create a host-microbe interface, arbuscular mycorrhizal (AM) fungi and rhizobia make use of the same endosymbiotic program. It is a long-standing hypothesis that pathogens make use of plant proteins that are dedicated to mutualistic symbiosis to infect plants and form haustoria. In this report, we developed a Phytophthora palmivora pathosystem to study haustorium formation in Medicago truncatula roots. We show that P. palmivora does not require host genes that are essential for symbiotic infection and host-microbe interface formation to infect Medicago roots and form haustoria. Based on these findings, we conclude that P. palmivora does not hijack the ancient intracellular accommodation program used by symbiotic microbes to form a biotrophic host-microbe interface.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genes, Plant
  • Host-Pathogen Interactions
  • Medicago truncatula / genetics
  • Medicago truncatula / microbiology*
  • Mycorrhizae / physiology*
  • Phytophthora / pathogenicity*
  • Plant Roots / microbiology*
  • Rhizobium / physiology*
  • Symbiosis*