Phylogenetic analysis of human group C rotavirus in hospitalized children with gastroenteritis in Belém, Brazil

J Med Virol. 2016 Apr;88(4):728-33. doi: 10.1002/jmv.24379. Epub 2015 Sep 28.

Abstract

Group C rotavirus (RVC) is potentially an important pathogen associated with acute gastroenteritis (AG), especially in outbreaks. This study aims to detect and molecularly characterize RVC in hospitalized children with AG in Belém, Brazil. From May 2008 to April 2011, 279 stools were subjected to reverse-transcription polymerase chain reaction targeting VP7, VP6, VP4, and NSP4 genes. RVC positivity rate was 2.1% (6/279) and phylogenetic analysis of positive samples yields genotype G4-P[2]-I2-E2. No evidence of zoonotic transmission and VP7 gene demonstrated close relationship with Asian strains. RVC surveillance is worth to expand information on evolutionary and epidemiological features of this virus.

Keywords: acute gastroenteritis; group C rotavirus; hospitalized children.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brazil / epidemiology
  • Child, Hospitalized
  • Child, Preschool
  • Feces / virology
  • Female
  • Gastroenteritis / epidemiology
  • Gastroenteritis / virology*
  • Genetic Variation*
  • Genotype*
  • Humans
  • Infant
  • Male
  • Molecular Epidemiology
  • Phylogeny*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rotavirus / classification*
  • Rotavirus / genetics*
  • Rotavirus / isolation & purification
  • Rotavirus Infections / epidemiology
  • Rotavirus Infections / virology*
  • Viral Nonstructural Proteins / genetics
  • Viral Structural Proteins / genetics

Substances

  • Viral Nonstructural Proteins
  • Viral Structural Proteins