From Structure to Function: A Comprehensive Compendium of Tools to Unveil Protein Domains and Understand Their Role in Cytokinesis

Methods Mol Biol. 2016:1369:379-92. doi: 10.1007/978-1-4939-3145-3_24.

Abstract

Unveiling the function of a novel protein is a challenging task that requires careful experimental design. Yeast cytokinesis is a conserved process that involves modular structural and regulatory proteins. For such proteins, an important step is to identify their domains and structural organization. Here we briefly discuss a collection of methods commonly used for sequence alignment and prediction of protein structure that represent powerful tools for the identification homologous domains and design of structure-function approaches to test experimentally the function of multi-domain proteins such as those implicated in yeast cytokinesis.

Keywords: Anillin; Cell division; Cytokinesis; Sad kinase; Structure-function analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Computational Biology / methods*
  • Cytokinesis*
  • Gene Expression
  • Models, Molecular*
  • Mutation
  • Protein Conformation*
  • Protein Interaction Domains and Motifs*
  • Proteins / chemistry*
  • Proteins / genetics
  • Quantitative Structure-Activity Relationship*
  • Sequence Alignment
  • Yeasts / genetics

Substances

  • Proteins