Establishment of human-embryonic-stem-cell line from mosaic trisomy 9 embryo

Boxian Huang; Chunyan Jiang; Aiqin Chen; Yugui Cui; Jiazi Xie; Jiandong Shen; Juan Chen; Lingbo Cai; Tingting Liao; Song Ning; Shi-Wen Jiang; Guoping Fan; Lianju Qin; Jiayin Liu

doi:10.1016/j.tjog.2015.08.003

Establishment of human-embryonic-stem-cell line from mosaic trisomy 9 embryo

Taiwan J Obstet Gynecol. 2015 Oct;54(5):505-11. doi: 10.1016/j.tjog.2015.08.003.

Authors

Boxian Huang¹, Chunyan Jiang², Aiqin Chen², Yugui Cui², Jiazi Xie², Jiandong Shen², Juan Chen², Lingbo Cai², Tingting Liao², Song Ning², Shi-Wen Jiang³, Guoping Fan⁴, Lianju Qin⁵, Jiayin Liu⁶

Affiliations

¹ State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210038, China.
² State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
³ Department of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA.
⁴ Department of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.
⁵ State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China. Electronic address: ljqin@njmu.edu.cn.
⁶ State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210038, China. Electronic address: jyliu_nj@126.com.

PMID: 26522100
DOI: 10.1016/j.tjog.2015.08.003

Abstract

Objective: Human-embryonic-stem-cell (hESC) lines derived from chromosomally or genetically abnormal embryos obtained following preimplantation genetic diagnosis are valuable in investigating genetic disorders.

Materials and methods: In this study, a new hESC line, Center of Clinical Reproductive Medicine 8 (CCRM8) was established by isolation, culture, and passaging of the inner cell mass of mosaic trisomy 9 embryos.

Results: A karyotype analysis showed that the hESC line possessed a euploid (46 chromosomes). The undifferentiated hESCs exhibited long-term proliferation capacity and expressed typical markers of OCT4, TRA-1-60, and TRA-1-81. In vitro embryoid-body (EB) formation, differentiation, and in vivo teratoma production confirmed the pluripotency of the hESC line. The data represented here are the first detailed report on the characterization and differentiation of one Chinese hESC line generated from mosaic trisomy 9 embryos.

Conclusion: Our study showed that chromosomally aberrant embryos could generate a normal hESC line, which would be useful in investigating gene function and embryo development.

Keywords: euploid recovery; human embryonic stem cells; mosaic trisomy 9.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blastocyst / cytology*
Cell Differentiation
Cell Line
Chromosomes, Human, Pair 9 / genetics
Embryo Culture Techniques
Embryonic Development / genetics*
Embryonic Stem Cells / cytology*
Female
Humans
Karyotype
Karyotyping
Pregnancy
Preimplantation Diagnosis / methods*
Trisomy / genetics*

Supplementary concepts

Chromosome 9, trisomy