Human Herpesvirus 6A U14 Is Important for Virus Maturation

Junko Mori; Huamin Tang; Akiko Kawabata; Masato Koike; Yasuko Mori

doi:10.1128/JVI.02492-15

Human Herpesvirus 6A U14 Is Important for Virus Maturation

J Virol. 2015 Nov 11;90(3):1677-81. doi: 10.1128/JVI.02492-15. Print 2016 Feb 1.

Authors

Junko Mori¹, Huamin Tang², Akiko Kawabata¹, Masato Koike³, Yasuko Mori⁴

Affiliations

¹ Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan.
² Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan Department of Immunology, Nanjing Medical University, Nanjing, China.
³ Departments of Cellular and Molecular Neuropathology, Juntendo University Graduate School of Medicine, Tokyo, Japan.
⁴ Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan ymori@med.kobe-u.ac.jp.

Abstract

Human herpesvirus 6A (HHV-6A) U14 is a virion protein with little known function in virus propagation. Here, we elucidated its function by constructing and analyzing U14-mutated viruses. We found that U14 is essential for HHV-6A propagation. We then constructed a mutant virus harboring dysfunctional U14. This virus showed severely reduced growth and retarded maturation. Taken together, these data indicate that U14 plays an important role during HHV-6A maturation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cells, Cultured
Herpesvirus 6, Human / genetics
Herpesvirus 6, Human / physiology*
Humans
Leukocytes, Mononuclear / virology
Mutant Proteins / genetics
Mutant Proteins / metabolism
Viral Structural Proteins / genetics
Viral Structural Proteins / metabolism*
Virus Replication*

Substances

Mutant Proteins
Viral Structural Proteins