Laboratory Evaluation of a Dual-Path Platform Assay for Rapid Point-of-Care HIV and Syphilis Testing

J Clin Microbiol. 2016 Feb;54(2):492-4. doi: 10.1128/JCM.03152-15. Epub 2015 Dec 9.

Abstract

We assessed the laboratory performance of the Chembio dual-path platform HIV-syphilis rapid immunodiagnostic test and electronic reader for detection of HIV and Treponema pallidum antibodies in 450 previously characterized serum specimens. For visual or electronic reader HIV antibody detection, the sensitivity was 100% and the specificity was 98.7%. For visual T. pallidum antibody detection, the test sensitivity was 94.7% and the specificity was 100.0%; with the electronic reader, the sensitivity was 94.7% and the specificity was 99.7%.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antigens, Bacterial / immunology
  • Coinfection*
  • HIV Antibodies / immunology
  • HIV Core Protein p24 / immunology
  • HIV Infections / diagnosis*
  • Humans
  • Immunologic Tests* / methods
  • Immunologic Tests* / standards
  • Point-of-Care Systems*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Syphilis / diagnosis*

Substances

  • Antigens, Bacterial
  • HIV Antibodies
  • HIV Core Protein p24