Subtype prediction in primary aldosteronism: measurement of circadian variation of adrenocortical hormones and 24-h urinary aldosterone

Clin Endocrinol (Oxf). 2016 Jun;84(6):814-21. doi: 10.1111/cen.12998. Epub 2016 Feb 4.

Abstract

Objective: Currently, adrenal venous sampling (AVS) is the only reliable method to distinguish unilateral from bilateral hyperaldosteronism in primary aldosteronism (PA). However, AVS is costly and time-consuming compared with simple blood tests. In this study, we conducted a retrospective study to determine whether circadian variation in plasma adrenocortical hormone levels (i.e. aldosterone, cortisol and ACTH) and a 24-h urinary aldosterone could contribute to the clinical differentiation between unilateral hyperaldosteronism (UHA) and bilateral hyperaldosteronism (BHA).

Design: In 64 patients who were diagnosed with PA and underwent AVS, 32 and 22 patients were diagnosed with UHA and BHA, respectively. Plasma adrenocortical hormone levels at 0:00, 6:00, 12:00 and 18:00 and 24-h urinary aldosterone under a condition of 6 g daily dietary sodium chloride intake were measured.

Results: Baseline plasma aldosterone concentration (PAC) and 24-h urinary aldosterone level in patients with UHA were significantly higher than in patients with BHA, particularly at 6:00. The area under the ROC curve for PAC at 0:00, 6:00, 12:00 and 18:00 and 24-h urinary aldosterone to discriminate UHA and BHA was 0·839 [95% confidence interval (CI); 0·73-0·95], 0·922 (95% CI; 0·85-1·00), 0·875 (95% CI; 0·78-0·97), 0·811 (95% CI; 0·69-0·93), 0·898 (95% CI; 0·81-0·99), respectively.

Conclusions: PAC at different blood sampling times and 24-h urinary aldosterone level may be diagnostically helpful in discriminating between UHA and BHA. We believe that these tests could reduce the number of unnecessary AVS procedures.

MeSH terms

  • Adrenocorticotropic Hormone / blood
  • Adult
  • Aldosterone / blood*
  • Aldosterone / urine*
  • Area Under Curve
  • Blood Specimen Collection
  • Circadian Rhythm
  • Diagnosis, Differential
  • Female
  • Humans
  • Hydrocortisone / blood
  • Hyperaldosteronism / diagnosis*
  • Male
  • Middle Aged
  • Retrospective Studies
  • Time Factors

Substances

  • Aldosterone
  • Adrenocorticotropic Hormone
  • Hydrocortisone