Target identification of novel therapeutic drugs is pivotal for the establishment of (1) new anticancer regiments, (2) to control side effects of the drugs, and (3) to identify appropriate combinations with established drugs. Here, we describe several in vitro assays applicable to characterize different characteristics of tumor cells. Furthermore, we present a protocol for establishing a reporter gene system for in vivo imaging, allowing for the study of drug effects in small animal models.
Keywords: Apoptosis; Cell cycle; FACS; Fluorescence; In vivo imaging; Tumor invasion.