Low Dose Infliximab for Prevention of Postoperative Recurrence of Crohn's Disease: Long Term Follow-Up and Impact of Infliximab Trough Levels and Antibodies to Infliximab

PLoS One. 2015 Dec 15;10(12):e0144900. doi: 10.1371/journal.pone.0144900. eCollection 2015.

Abstract

Objective: In patients with postoperative recurrence of Crohn's disease endoscopic and clinical remission can be maintained for up to 1 year with low infliximab doses (3 mg/Kg). However, in theory low-dose infliximab treated patients could develop subtherapeutic trough levels, infiximab antibodies, and might loose response to therapy. To verify this hypothesis infliximab pharmacokinetics and clinical/endoscopic response were checked in a group of patients treated in the long term with low infliximab doses.

Design: Infliximab antibodies, infliximab levels, highly-sensitive CRP and fecal calprotectin were measured during the 8-week interval in 5 consecutive patients in clinical (Crohn's Disease Activity Index < 150) and endoscopic (Rutgeerts scores 0-1) remission after one year of therapy with infliximab 3 mg/Kg. For comparison with reported standards, infliximab pharmacokinetics and inflammatory parameters were also tested in 6 Crohn's disease patients who did not undergo surgery and who were in clinical remission while on infliximab 5 mg/Kg. Patients on low infliximab dose also underwent colonoscopy after 18 additional months of therapy.

Results: Highly sensitive CRP and fecal calprotectin increased in all patients during the 8-week interval. Infliximab trough levels were lower in patients treated with the low dose compared to controls (mean±SE: 2.0±0.3 vs 4.75±0.83 μg/mL respectively p<0.05). Infliximab antibodies were present in two of the subjects treated with low infliximab dose and in none of the controls. However, in low dose-treated patients after 18 additional months of therapy endoscopy continued to show mucosal remission and none of them developed clinical recurrence or side effects.

Conclusions: Patients treated with low infliximab doses had lower trough levels compared to patients treated with 5 mg/Kg and some developed antibodies to infliximab. However, low infliximab doses sustained clinical and endoscopic remission for a total of 30 months of treatment.

MeSH terms

  • Adult
  • Biomarkers / metabolism
  • C-Reactive Protein / metabolism
  • Case-Control Studies
  • Crohn Disease / drug therapy*
  • Crohn Disease / prevention & control
  • Crohn Disease / surgery*
  • Dose-Response Relationship, Drug
  • Endoscopy
  • Feces / chemistry
  • Female
  • Follow-Up Studies
  • Humans
  • Infliximab / immunology*
  • Infliximab / pharmacokinetics
  • Infliximab / therapeutic use*
  • Leukocyte L1 Antigen Complex / metabolism
  • Male
  • Middle Aged
  • Postoperative Period
  • Recurrence

Substances

  • Biomarkers
  • Leukocyte L1 Antigen Complex
  • C-Reactive Protein
  • Infliximab

Grants and funding

The authors have no support or funding to report.