Formation of Cholangiogenic Cysts Following Intrahepatic Islet Transplantation in Streptozotocin Diabetic Rats

Transplant Proc. 2015 Nov;47(9):2763-7. doi: 10.1016/j.transproceed.2015.07.032.

Abstract

Permanent hyperinsulinemia and the resulting overstimulation of the insulin receptor signaling pathway is suspected as a trigger of cancer genesis in the livers of type 2 diabetic patients. Liver tissue (LT) surrounding transplanted pancreatic islets (PI) can be permanently exposed to insulin in even higher concentrations than in type 2 diabetic patients. Therefore, this study examines the effect of PI transplantation (Tx) on LT in animals with streptozotocin (STZ)-induced diabetes mellitus. The suboptimal mass (400 or 1000) of isogeneic PI was transplanted into either the portal vein or under the kidney capsule of diabetic Brown Norway (BN) rats. Healthy BN rats treated with 400 isogeneic PI transplanted in the portal vein served as a control group. During the first 6 months after PI Tx, small and infrequent cystic lesions developed in animals with STZ diabetes, irrespective of the Tx site. In 10 months, frequent and complex cystic lesions appeared in these animals. In the control group, several small lesions were detected but not until 10 months after the PI Tx. In summary, STZ is the likely main inductor of hepatic cystic lesions, but the contribution of PI was not confirmed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cysts / etiology*
  • Diabetes Mellitus, Experimental / complications*
  • Insulin / metabolism*
  • Islets of Langerhans / pathology
  • Islets of Langerhans Transplantation / adverse effects*
  • Liver Neoplasms / etiology*
  • Liver*
  • Male
  • Portal Vein / surgery
  • Rats
  • Rats, Inbred BN
  • Streptozocin
  • Transplantation, Heterotopic*

Substances

  • Insulin
  • Streptozocin