Preclinical Assessment of a Fully Automated Multiplex PCR Panel for Detection of Central Nervous System Pathogens

J Clin Microbiol. 2016 Mar;54(3):785-7. doi: 10.1128/JCM.02850-15. Epub 2015 Dec 30.

Abstract

We evaluated a multiplexed PCR panel for the detection of 16 bacterial, viral, and fungal pathogens in cerebrospinal fluid. Panel results were compared to routine testing, and discrepancies were resolved by additional nucleic acid amplification tests or sequencing. Overall, the positive and negative agreements across methods were 92.9% and 91.9%, respectively.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Automation*
  • Central Nervous System Infections / diagnosis*
  • Central Nervous System Infections / etiology*
  • Humans
  • Multiplex Polymerase Chain Reaction* / methods
  • Reagent Kits, Diagnostic
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Reagent Kits, Diagnostic