Titanium Implant Impairment and Surrounding Muscle Cell Death Following High-Salt Diet: An In Vivo Study

PLoS One. 2016 Jan 13;11(1):e0146873. doi: 10.1371/journal.pone.0146873. eCollection 2016.

Abstract

Aim of the study: High-salt consumption has been widely described as a risk factor for cardiovascular, renal and bone functions. In the present study, the extent to which high-salt diet could influence Ti6Al4V implant surface characteristic, its adhesion to rat tibial crest, and could modify muscle cell viability of two surrounding muscles, was investigated in vivo. These parameters have also been assessed following a NMES (neuro-myoelectrostimulation) program similar to that currently used in human care following arthroplasty.

Results: After a three-week diet, a harmful effect on titanium implant surface and muscle cell viability was noted. This is probably due to salt corrosive effect on metal and then release of toxic substance around biologic tissue. Moreover, if the use of NMES with high-salt diet induced muscles damages, the latter were higher when implant was added. Unexpectedly, higher implant-to-bone adhesion was found for implanted animals receiving salt supplementation.

Conclusion: Our in vivo study highlights the potential dangerous effect of high-salt diet in arthroplasty based on titanium prosthesis. This effect appears to be more important when high-salt diet is combined with NMES.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alloys
  • Animals
  • Arthroplasty
  • Blood Pressure
  • Cell Survival
  • Coated Materials, Biocompatible
  • Diet
  • Electric Stimulation
  • Male
  • Muscles / pathology
  • Muscles / physiology*
  • Osseointegration / physiology
  • Prostheses and Implants*
  • Prosthesis Implantation
  • Rats
  • Rats, Sprague-Dawley
  • Risk Factors
  • Sodium Chloride, Dietary / adverse effects*
  • Surface Properties
  • Tibia / pathology
  • Titanium / chemistry*

Substances

  • Alloys
  • Coated Materials, Biocompatible
  • Sodium Chloride, Dietary
  • titanium alloy (TiAl6V4)
  • Titanium

Grants and funding

This work was supported by public grants of Aix-Marseille Université (AMU) and Centre National de la Recherche Scientifique (CNRS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.