Abstract
Objective:
We hypothesized that the hypoxia-inducible factor (HIF) 1α in vascular smooth muscle contributes to the development of atherosclerosis, and links intravascular pressure to this process.
Approach and results:
Transverse aortic constriction was used to create high-pressure vascular segments in control, apolipoprotein E (ApoE)(-/-), smooth muscle-HIF1α(-/-), and ApoE(-/-)×smooth muscle-HIF1α(-/-) double-knockout mice. Transverse aortic constriction selectively induced atherosclerosis in high-pressure vascular segments in young ApoE(-/-) mice on normal chow, including coronary plaques within 1 month. Concomitant deletion of HIF1α from smooth muscle significantly reduced vascular inflammation, and attenuated atherosclerosis.
Conclusions:
HIF1α in vascular smooth muscle plays an important role in the pathogenesis of atherosclerosis, and may provide a mechanistic link between blood pressure, vascular inflammation, and lipid deposition.
Keywords:
atherosclerosis; blood pressure; hypoxia-inducible factor; inflammation; smooth muscle.
© 2016 American Heart Association, Inc.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Aorta / metabolism
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Aorta / physiopathology
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Aortic Diseases / genetics
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Aortic Diseases / metabolism*
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Aortic Diseases / pathology
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Aortic Diseases / physiopathology
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Aortic Diseases / prevention & control
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Apolipoproteins E / deficiency
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Apolipoproteins E / genetics
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Arterial Pressure*
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Atherosclerosis / genetics
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Atherosclerosis / metabolism*
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Atherosclerosis / pathology
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Atherosclerosis / physiopathology
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Atherosclerosis / prevention & control
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Blood Flow Velocity
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Diet, High-Fat
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Disease Models, Animal
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Gene Expression Regulation
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Hypoxia-Inducible Factor 1, alpha Subunit / deficiency
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Hypoxia-Inducible Factor 1, alpha Subunit / genetics
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Hypoxia-Inducible Factor 1, alpha Subunit / metabolism*
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Ligation
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Mice, Inbred C57BL
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Mice, Knockout
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Muscle, Smooth, Vascular / metabolism*
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Muscle, Smooth, Vascular / pathology
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Muscle, Smooth, Vascular / physiopathology
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Muscle, Smooth, Vascular / surgery
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Plaque, Atherosclerotic
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Regional Blood Flow
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Signal Transduction
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Time Factors
Substances
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Apolipoproteins E
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Hif1a protein, mouse
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Hypoxia-Inducible Factor 1, alpha Subunit