Quantitative assessments of glycolysis from single cells

Young Shik Shin; Jungwoo Kim; Dazy Johnson; Alex A Dooraghi; Wilson X Mai; Lisa Ta; Arion F Chatziioannou; Michael E Phelps; David A Nathanson; James R Heath

doi:10.1142/S2339547815200058

Quantitative assessments of glycolysis from single cells

Technology (Singap World Sci). 2015 Jun;3(4):172-178. doi: 10.1142/S2339547815200058. Epub 2015 Jun 25.

Authors

Affiliations

¹ Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA; NanoSystems Biology Cancer Center, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
² NanoSystems Biology Cancer Center, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
³ Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
⁴ Crump Institute for Molecular Imaging, University of California, Los Angeles, CA 90095, USA.
⁵ Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA; Crump Institute for Molecular Imaging, University of California, Los Angeles, CA 90095, USA; NanoSystems Biology Cancer Center, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.

Abstract

The most common positron emission tomography (PET) radio-labeled probe for molecular diagnostics in patient care and research is the glucose analog, 2-deoxy-2-[F-18]fluoro-D-glucose (¹⁸F-FDG). We report on an integrated microfluidics-chip/beta particle imaging system for in vitro¹⁸F-FDG radioassays of glycolysis with single cell resolution. We investigated the kinetic responses of single glioblastoma cancer cells to targeted inhibitors of receptor tyrosine kinase signaling. Further, we find a weak positive correlation between cell size and rate of glycolysis.

Abstract

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