Visual Assay of Total Iron in Human Serum with Bathophenanthrolin Disulfonate-accommodated MCM-41

Anal Sci. 2016;32(2):241-4. doi: 10.2116/analsci.32.241.

Abstract

A simple visual method for determining the total iron in human serum is proposed based on color development in the nanospace of mesoporous silica MCM-41 and a chromogenic ligand bathophenathroline disulfonate (BPS). Observing the color intensity of a complex between iron(II) and BPS devloped on the MCM-41 material by the naked eye enabled us to quntify iron(II) with a detection limit of 0.5 μM. The BPS-loaded MCM-41 was successfully applied for quantifying the total iron in human serum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Adult
  • Calibration
  • Color
  • Dose-Response Relationship, Drug
  • Humans
  • Iron / blood*
  • Limit of Detection
  • Lipids / chemistry
  • Male
  • Phenanthrolines / chemistry*
  • Propylamines / chemistry
  • Serum Albumin / chemistry
  • Silanes / chemistry
  • Silicon Dioxide / chemistry*

Substances

  • Lipids
  • MCM-41
  • Phenanthrolines
  • Propylamines
  • Serum Albumin
  • Silanes
  • 3-aminopropyltrimethoxysilane
  • bathophenanthroline disulfonic acid
  • Silicon Dioxide
  • Iron