Distinguishing Lung Adenocarcinoma from Lung Squamous Cell Carcinoma by Two Hypomethylated and Three Hypermethylated Genes: A Meta-Analysis

Tao Huang; Jinyun Li; Cheng Zhang; Qingxiao Hong; Danjie Jiang; Meng Ye; Shiwei Duan

doi:10.1371/journal.pone.0149088

Distinguishing Lung Adenocarcinoma from Lung Squamous Cell Carcinoma by Two Hypomethylated and Three Hypermethylated Genes: A Meta-Analysis

PLoS One. 2016 Feb 10;11(2):e0149088. doi: 10.1371/journal.pone.0149088. eCollection 2016.

Authors

Tao Huang¹, Jinyun Li¹, Cheng Zhang¹, Qingxiao Hong¹, Danjie Jiang¹, Meng Ye², Shiwei Duan¹

Affiliations

¹ Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
² The Affiliated Hospital, Ningbo University, Ningbo, Zhejiang 315000, China.

Abstract

Significant differences in the aberrant methylation of genes exist among various histological types of non-small cell lung cancer (NSCLC), which includes adenocarcinoma (AC) and squamous cell carcinoma (SCC). Different chemotherapeutic regimens should be administered to the two NSCLC subtypes due to their unique genetic and epigenetic profiles. The purpose of this meta-analysis was to generate a list of differentially methylated genes between AC and SCC. Our meta-analysis encompassed 151 studies on 108 genes among 12946 AC and 10243 SCC patients. Our results showed two hypomethylated genes (CDKN2A and MGMT) and three hypermethylated genes (CDH13, RUNX3 and APC) in ACs compared with SCCs. In addition, our results showed that the pooled specificity and sensitivity values of CDH13 and APC were higher than those of CDKN2A, MGMT and RUNX3. Our findings might provide an alternative method to distinguish between the two NSCLC subtypes.

Publication types

Meta-Analysis
Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / diagnosis*
Adenocarcinoma / genetics
Adenomatous Polyposis Coli Protein / genetics
Cadherins / genetics
Carcinoma, Non-Small-Cell Lung / diagnosis*
Carcinoma, Non-Small-Cell Lung / genetics
Carcinoma, Squamous Cell / diagnosis*
Carcinoma, Squamous Cell / genetics
Core Binding Factor Alpha 3 Subunit / genetics
Cyclin-Dependent Kinase Inhibitor p16 / genetics
DNA Methylation*
DNA Modification Methylases / genetics
DNA Repair Enzymes / genetics
Databases, Genetic
Diagnosis, Differential*
Epigenesis, Genetic
Humans
Lung Neoplasms / diagnosis*
Lung Neoplasms / genetics
Sensitivity and Specificity
Tumor Suppressor Proteins / genetics

Substances

APC protein, human
Adenomatous Polyposis Coli Protein
Cadherins
Core Binding Factor Alpha 3 Subunit
Cyclin-Dependent Kinase Inhibitor p16
H-cadherin
Runx3 protein, human
Tumor Suppressor Proteins
DNA Modification Methylases
MGMT protein, human
DNA Repair Enzymes

Grants and funding

The research was supported by the grants from the National Natural Science Foundation of China (31100919 and 81371469), the Natural Science Foundation of Zhejiang Province (LR13H020003), the K. C. Wong Magna Fund in Ningbo University, the Zhejiang Provincial Natural Science Foundation of China (LY16H160005), Project of Scientific Innovation Team of Ningbo (2015B11050) and the Ningbo Natural Science Foundation (2014A610235). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.