A sensitive fluorescent sensor for the detection of endogenous hydroxyl radicals in living cells and bacteria and direct imaging with respect to its ecotoxicity in living zebra fish

Chem Commun (Camb). 2016 Mar 28;52(25):4636-9. doi: 10.1039/c5cc10658c.

Abstract

We have synthesized a novel fluorescent probe, , which shown a high potential for imaging of endogenous ˙OH in living cells and various types of bacteria. In addition, it is an excellent sensor for in vivo imaging of ˙OH generated following treatment with TiO2NPs in zebra fish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteria / chemistry*
  • Bacteria / drug effects*
  • Cell Survival / drug effects
  • Embryo, Nonmammalian / chemistry
  • Embryo, Nonmammalian / drug effects*
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Hydroxyl Radical / analysis*
  • Hydroxyl Radical / toxicity*
  • Indoles / chemistry*
  • Limit of Detection
  • Molecular Structure
  • Nanoparticles / chemistry
  • Phenothiazines / chemistry*
  • Titanium / chemistry
  • Zebrafish

Substances

  • Fluorescent Dyes
  • Indoles
  • MPT-Cy2
  • OMPT-Cy2
  • Phenothiazines
  • titanium dioxide
  • Hydroxyl Radical
  • Titanium