Complement factor H binding of monomeric C-reactive protein downregulates proinflammatory activity and is impaired with at risk polymorphic CFH variants

Sci Rep. 2016 Mar 10:6:22889. doi: 10.1038/srep22889.

Abstract

Inflammation and immune-mediated processes are pivotal to the pathogenic progression of age-related macular degeneration (AMD). Although plasma levels of C-reactive protein (CRP) have been shown to be associated with an increased risk for AMD, the pathophysiological importance of the prototypical acute-phase reactant in the etiology of the disease is unknown, and data regarding the exact role of CRP in ocular inflammation are limited. In this study, we provide mechanistic insight into how CRP contributes to the development of AMD. In particular, we show that monomeric CRP (mCRP) but not the pentameric form (pCRP) upregulates IL-8 and CCL2 levels in retinal pigment epithelial cells. Further, we show that complement factor H (FH) binds mCRP to dampen its proinflammatory activity. FH from AMD patients carrying the "risk" His402 polymorphism displays impaired binding to mCRP, and therefore proinflammatory effects of mCRP remain unrestrained.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • C-Reactive Protein / metabolism*
  • Cell Line
  • Chemotaxis, Leukocyte
  • Complement Factor H / chemistry
  • Complement Factor H / genetics
  • Complement Factor H / metabolism*
  • Cytokines / metabolism
  • Down-Regulation
  • Female
  • Genetic Predisposition to Disease
  • Humans
  • Inflammation / metabolism
  • Interleukin-8 / metabolism
  • Leukocytes, Mononuclear / physiology
  • Male
  • Polymorphism, Genetic
  • Protein Binding
  • Protein Multimerization
  • Retinal Pigment Epithelium / metabolism*
  • Wet Macular Degeneration / genetics
  • Wet Macular Degeneration / metabolism

Substances

  • Cytokines
  • Interleukin-8
  • Complement Factor H
  • C-Reactive Protein