Genetic variants of lncRNA HOTAIR contribute to the risk of osteosarcoma

Oncotarget. 2016 Apr 12;7(15):19928-34. doi: 10.18632/oncotarget.7957.

Abstract

Osteosarcoma (OS) is the most common primary malignant bone tumor in adolescents and young adults. However, the essential mechanisms underlying osteosarcomagenesis remain obscure. The HOTAIR, a well-known long noncoding RNA (lncRNA), is involved in pathogenesis and progress of multiple tumors. To reveal the potential role of lncRNA HOTAIR in OS carcinogenesis, we conducted a two-stage, case-control study among Chinese population with 900 OS cases and 900 controls to evaluated associations of its genetic variants with OS risk. We found that C allele of rs7958904 was associated with a significantly decreased OS risk when compared with G allele (OR: 0.77; 95% CI: 0.67-0.90; P = 6.77×10-4). Functional analyses on HOTAIR Expression showed that the expression level of HOTAIR in OS tissues was significantly higher than that in corresponding normal tissues, and subjects with the rs7958904 CC genotype had significantly lower HOTAIR RNA levels than those of other genotypes. This should be the first study to examine the association between HOTAIR variants and OS risk.

Keywords: HOTAIR; lncRNA; osteosarcoma; susceptibility; variant.

MeSH terms

  • Adult
  • Asian People
  • Biomarkers, Tumor / genetics*
  • Bone Neoplasms / genetics*
  • Bone Neoplasms / pathology
  • Case-Control Studies
  • Female
  • Follow-Up Studies
  • Gene Expression Regulation, Neoplastic
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • Male
  • Neoplasm Staging
  • Osteosarcoma / genetics*
  • Osteosarcoma / pathology
  • Polymorphism, Single Nucleotide*
  • Prognosis
  • RNA, Long Noncoding / genetics*
  • Risk Factors
  • Young Adult

Substances

  • Biomarkers, Tumor
  • HOTAIR long untranslated RNA, human
  • RNA, Long Noncoding