Polymorphisms of Selected DNA Repair Genes and Lung Cancer in Chromium Exposure

Adv Exp Med Biol. 2016:911:17-22. doi: 10.1007/5584_2016_218.

Abstract

Chromium is a well-known mutagen and carcinogen involved in lung cancer development. DNA repair genes play an important role in the elimination of genetic changes caused by chromium exposure. In the present study, we investigated the polymorphisms of the following DNA repair genes: XRCC3, participating in the homologous recombination repair, and hMLH1 and hMSH2, functioning in the mismatch repair. We focused on the risk the polymorphisms present in the development of lung cancer regarding the exposure to chromium. We analyzed 106 individuals; 45 patients exposed to chromium with diagnosed lung cancer and 61 healthy controls. Genotypes were determined by a PCR-RFLP method. We unravelled a potential for increased risk of lung cancer development in the hMLH1 (rs1800734) AA genotype in the recessive model. In conclusion, gene polymorphisms in the DNA repair genes underscores the risk of lung cancer development in chromium exposed individuals.

Keywords: Carcinogens; Chromium exposure; DNA repair; Genes; Genotype; Lung cancer; Polymorphism.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Case-Control Studies
  • Chromium / adverse effects*
  • DNA Repair
  • DNA-Binding Proteins / genetics*
  • Female
  • Follow-Up Studies
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • Lung Neoplasms / chemically induced
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology
  • Male
  • Middle Aged
  • MutL Protein Homolog 1 / genetics*
  • MutS Homolog 2 Protein / genetics*
  • Neoplasm Staging
  • Occupational Exposure / adverse effects*
  • Polymorphism, Genetic / genetics*
  • Prognosis
  • Risk Factors
  • Survival Rate

Substances

  • DNA-Binding Proteins
  • MLH1 protein, human
  • X-ray repair cross complementing protein 3
  • Chromium
  • MSH2 protein, human
  • MutL Protein Homolog 1
  • MutS Homolog 2 Protein