Opportunities and limitations for combined fly-scan ptychography and fluorescence microscopy

Proc SPIE Int Soc Opt Eng. 2015 Aug 9:9592:95920U. doi: 10.1117/12.2190749. Epub 2015 Sep 18.

Abstract

X-ray fluorescence offers unparalleled sensitivity for imaging the nanoscale distribution of trace elements in micrometer thick samples, while x-ray ptychography offers an approach to image light element containing structures at a resolution beyond that of the x-ray lens used. These methods can be used in combination, and in continuous scan mode for rapid data acquisition when using multiple probe mode reconstruction methods. We discuss here the opportunities and limitations of making use of additional information provided by ptychography to improve x-ray fluorescence images in two ways: by using position-error-correction algorithms to correct for scan distortions in fluorescence scans, and by considering the signal-to-noise limits on previously-demonstrated ptychographic probe deconvolution methods. This highlights the advantages of using a combined approach.

Keywords: Ptychography; deconvolution; distortion correction; fluorescence microscopy; fly scan; multiple probe modes.