Comparison of Culture-Based Methods for Identification of Colonization with Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus in the Context of Cocolonization

Meghan F Davis; Baofeng Hu; Karen C Carroll; Warren B Bilker; Pam Tolomeo; Valerie C Cluzet; Patrick Baron; Jacqueline M Ferguson; Daniel O Morris; Shelley C Rankin; Ebbing Lautenbach; Irving Nachamkin

doi:10.1128/JCM.00132-16

Comparison of Culture-Based Methods for Identification of Colonization with Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus in the Context of Cocolonization

J Clin Microbiol. 2016 Jul;54(7):1907-1911. doi: 10.1128/JCM.00132-16. Epub 2016 Apr 27.

Authors

Affiliations

¹ Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA mdavis65@jhu.edu nachamki@upenn.edu.
² Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
³ Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
⁴ Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
⁵ Center for Clinical Epidemiology and Biostatistics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
⁶ Division of Infectious Diseases, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
⁷ Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
⁸ Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
⁹ Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
¹⁰ Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA mdavis65@jhu.edu nachamki@upenn.edu.

Abstract

Two screening methods to detect staphylococcal colonization in humans were compared. Direct plating to CHROMagar (BD Diagnostics) was compared to a broth preenrichment followed by plating to Baird-Parker agar. The broth-enrichment method was comparable to CHROMagar for methicillin-resistant Staphylococcus aureas (MRSA) detection, but the enrichment method was optimum for recovery of coagulase-positive Staphylococcus spp.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Bacteriological Techniques / methods*
Carrier State / diagnosis*
Carrier State / microbiology
Culture Media / chemistry
Humans
Mass Screening / methods*
Methicillin Resistance*
Staphylococcal Infections / diagnosis*
Staphylococcal Infections / microbiology
Staphylococcus aureus / drug effects*
Staphylococcus aureus / isolation & purification*

Substances

Culture Media

Abstract

Publication types

MeSH terms

Substances

Grants and funding