Abstract
Adult stem cells (SCs) are essential for tissue maintenance and regeneration yet are susceptible to senescence during aging. We demonstrate the importance of the amount of the oxidized form of cellular nicotinamide adenine dinucleotide (NAD(+)) and its effect on mitochondrial activity as a pivotal switch to modulate muscle SC (MuSC) senescence. Treatment with the NAD(+) precursor nicotinamide riboside (NR) induced the mitochondrial unfolded protein response and synthesis of prohibitin proteins, and this rejuvenated MuSCs in aged mice. NR also prevented MuSC senescence in the mdx (C57BL/10ScSn-Dmd(mdx)/J) mouse model of muscular dystrophy. We furthermore demonstrate that NR delays senescence of neural SCs and melanocyte SCs and increases mouse life span. Strategies that conserve cellular NAD(+) may reprogram dysfunctional SCs and improve life span in mammals.
Copyright © 2016, American Association for the Advancement of Science.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cellular Reprogramming
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Cellular Senescence / drug effects*
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Cellular Senescence / physiology
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Disease Models, Animal
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Longevity / drug effects*
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Longevity / physiology
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Melanocytes / cytology
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Melanocytes / drug effects
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Melanocytes / physiology
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Mice
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Mice, Inbred C57BL
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Mice, Inbred mdx
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Mitochondria / drug effects*
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Mitochondria / physiology
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Muscular Dystrophies / therapy
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Myoblasts, Skeletal / cytology
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Myoblasts, Skeletal / drug effects*
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Myoblasts, Skeletal / physiology
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NAD / metabolism
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NAD / pharmacology*
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Neural Stem Cells / cytology
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Neural Stem Cells / drug effects
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Neural Stem Cells / physiology
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Niacinamide / analogs & derivatives
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Niacinamide / metabolism
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Oxidative Stress
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Prohibitins
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Pyridinium Compounds
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Repressor Proteins / biosynthesis
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Unfolded Protein Response / drug effects
Substances
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Prohibitins
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Pyridinium Compounds
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Repressor Proteins
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nicotinamide-beta-riboside
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NAD
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Niacinamide