Induction Effect of Bisphenol A on Gene Expression Involving Hepatic Oxidative Stress in Rat

Oxid Med Cell Longev. 2016:2016:6298515. doi: 10.1155/2016/6298515. Epub 2016 Apr 10.

Abstract

Background and Objective. Bisphenol A (BPA) is an abundantly used xenoestrogenic chemical which may cause various disorders in body. In the present study, we sought to investigate the effects of various doses of BPA on hepatic oxidative stress-related gene expression in rats. Methods. Male Wistar rats weighing 150-200 g were used in this study. Three doses of the BPA (5, 25, and 125 μg/kg) in corn oil were administered as gavage during 35 consecutive days. After the experiment, the rats were expired and the livers were removed and stored at -80°C freezer for RNA extraction. Findings. The Real Time PCR showed increased expression of HO-1 in the rats receiving BPA doses compared to the control group. This effect was dose-dependent and higher at doses of 25 and 125 μg/kg than 5 μg/kg of body weight (p < 0.05). It was also demonstrated that various doses BPA can increase GADD45B gene expression compared to control group. That expression was significantly dominant in the lowest dose (5 μg/kg) of the BPA (p < 0.05). The final body weights (168.0 ± 10.0 gr) in the treatment group [BPA (125 μg/kg)] showed a significant decrease compared to control group (191.60 ± 6.50 gr). Conclusion. These findings demonstrate that BPA generated ROS and increased the antioxidant gene expression that causes hepatotoxicity.

MeSH terms

  • Animals
  • Antigens, Differentiation / metabolism
  • Benzhydryl Compounds / toxicity*
  • Body Weight / drug effects
  • Gene Expression Regulation / drug effects*
  • Heme Oxygenase-1 / metabolism
  • Liver / drug effects
  • Liver / metabolism*
  • Liver / pathology*
  • Male
  • Oxidative Stress / drug effects
  • Oxidative Stress / genetics*
  • Phenols / toxicity*
  • Rats, Wistar
  • Real-Time Polymerase Chain Reaction

Substances

  • Antigens, Differentiation
  • Benzhydryl Compounds
  • Gadd45b protein, rat
  • Phenols
  • Heme Oxygenase-1
  • bisphenol A