An epidermis-driven mechanism positions and scales stem cell niches in plants

Sci Adv. 2016 Jan 29;2(1):e1500989. doi: 10.1126/sciadv.1500989. eCollection 2016 Jan.

Abstract

How molecular patterning scales to organ size is highly debated in developmental biology. We explore this question for the characteristic gene expression domains of the plant stem cell niche residing in the shoot apical meristem. We show that a combination of signals originating from the epidermal cell layer can correctly pattern the key gene expression domains and notably leads to adaptive scaling of these domains to the size of the tissue. Using live imaging, we experimentally confirm this prediction. The identified mechanism is also sufficient to explain de novo stem cell niches in emerging flowers. Our findings suggest that the deformation of the tissue transposes meristem geometry into an instructive scaling and positional input for the apical plant stem cell niche.

Keywords: Arabidopsis thaliana; CLAVATA3; Computational Morphodynamics; Shoot apical meristem; Stem cells; WUSCHEL.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Epidermal Cells*
  • Epidermis / metabolism*
  • Flowers / metabolism
  • Gene Expression Regulation, Plant
  • Meristem / genetics
  • Meristem / metabolism
  • Plant Physiological Phenomena*
  • Signal Transduction
  • Stem Cell Niche*
  • Stem Cells / cytology
  • Stem Cells / metabolism