Pancreatic cancer screening using a multiplatform human serum metabolomics system

Biomark Med. 2016 Jun;10(6):577-86. doi: 10.2217/bmm-2016-0020. Epub 2016 May 12.

Abstract

Aim: To examine a novel screening method for pancreatic cancer involving gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry-based metabolomics analysis.

Materials & methods: Sera from pancreatic cancer patients (n = 59) and healthy volunteers (n = 59) were allocated to the training set or validation set. Serum metabolome analysis was carried out using our multiplatform metabolomics system. A diagnostic model was constructed using a two-phase screening method that was newly advocated.

Results: When the training set was used, the constructed diagnostic model exhibited high sensitivity (100%) and specificity (80%) for pancreatic cancer. When the validation set was used, the model displayed high sensitivity (84.1%) and specificity (84.1%).

Conclusion: We successfully developed a diagnostic model for pancreatic cancer using a multiplatform serum metabolomics system.

Keywords: biomarker; gas chromatography/mass spectrometry (GC/MS); liquid chromatography/mass spectrometry (LC/MS); metabolomics; pancreatic cancer; screening method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Area Under Curve
  • Biomarkers, Tumor / blood*
  • Case-Control Studies
  • Chromatography, High Pressure Liquid
  • Female
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Male
  • Mass Spectrometry
  • Metabolomics*
  • Middle Aged
  • Neoplasm Staging
  • Pancreatic Neoplasms / diagnosis*
  • Pancreatic Neoplasms / pathology
  • ROC Curve
  • Sensitivity and Specificity

Substances

  • Biomarkers, Tumor